David Skurnik

David Skurnik is professor at the Medical School Paris Descartes and Team Leader Escherichia coli in the INSERM research unit pathogenesis of systemic infections (INEM). A major goal of DS research is to use High-Throughput Sequencing (TnSeq) and a new approach called TnVaccine to understand the bacterial and host factors involved in pathogenesis and immunity to neonatal bacterial meningitis, focusing on Escherichia coli K1, and to reveal alternative approaches to current antibiotic treatments, with the prospect of a multivalent vaccine against this pathogen. In parallel, David Skurnik is also strongly involved in more clinically advanced projects using active and passive immunization against microbial pathogens, especially antibody conjugated antimicrobial peptide (ADC) against Pseudomonas aeruginosa, mRNA encoded antibodies (targeting P. aeruginosa and other Multi-drug resistant bacteria, and more recently SARS-CoV-2) and broad-spectrum antibodies (against MDR bacteria with the PNAG, Poly-Beta 1-6 N-Acetyl Glucosamine, as a vaccine target and against Influenza Viruses).



Submited and In Preparation manuscripts

COVID-19 pandemic: Impact caused by school closure and national lockdown on pediatric visits and admissions for viral and non-viral infections, a time series analysis
François Angoulvant, M.D., Ph.D., Naïm Ouldalu, M.D., David Dawei Yang,M.D., Mathilde Filser, Vincent Gajdos, M.D.,Ph.D. Alexis Rybak,M.D., Romain Guedj,M.D.,Ph.D., Valérie Soussan-Bannini,M.D., Romain Basmaci,M.D.,Ph.D., Alain Lefevre-Utile,M.D, Dominique Brun-Ney,M.D., Laure Beaujouan, David Skurnik,M.D.,Ph.D
Clinical Infectious Diseases in press

A high-throughput sequencing approach identifies immunotherapeutic targets for bacterial meningitis in neonates
Stephanie Pons, Damien Roux, Eric Frapy, Charlotte Gaultier, François Lebreton, Andrea Kropec, Olga Danilchanka, Laura Schlemmer, Hugues Aschard, Harry Sokol, Colette Cywes-Bentley, John J. Mekalanos, Thomas Guillard, Gerald B. Pier, David Skurnik
Revised version in preparation

A mechanism for aminoglycosides-mediated SOS induction in Escherichia coli that cross-select for fluoroquinolone resistance
Anamaria Babosan, David Skurnik, Anaëlle Muggeo, Gerald B. Pier, Fany Reffuveille, Frédéric Klein, Zeynep Baharoglu, Didier Mazel, Thomas Jové, Marie-Cécile Ploy, François Lebreton9 Sébastien Vergnolle, Christophe de Champs and Thomas Guillard
Revised version in preparation

Chlorine solutions for an efficient and safe method of decontamination of breast pump milk collection kits during and after the COVID-19 pandemic
V. Rigourd , M Benali, J Poupon ,J. Langrand , A Goutard, C Droguet, E Bille, P Frange, Y Bahri, D Pasquier, A Lapillonne, D Skurnik
Submitted

Development of an antibody fused with an antimicrobial peptide targeting Pseudomonas aeruginosa: a new approach to prevent and treat bacterial infections
Johnson, Kenneth; Delaney, James; Guillard, Thomas; Li, Kai; Wollacott, Andrew; Frapy, Eric; Mong, Surin; Tissire, Hamid; Viswanathan, Karthik; Touti, Faycal; Babcock, Gregory J; Shriver, Zachary; Penulute, Bradley L.; Plante, Obadiah1, and Skurnik, David
Submitted

Identification of an antibody recognizing the conserved inner core of the LPS of Pseudomonas aeruginosa
Elli, Stefano; Alekseeva, Anna; Ramakrishnan, Boopathy; Koch, Tyree; Viswanathan, Karthik;  Li, Kai; Shriver, Zachary; Skurnik David, Plante, Obadiah, and Guerrini, Marco
Submitted


Grants and Funding

Call, funding agency, grant allocatedProject’s titleName of the scientific coordinatorStart - End
Research agreement with Visterra Inc
(180,000 euros)
Antibody-drug conjugate to treat infections caused by Pseudomonas aeruginosaSkurnik David2018-2021
R01, NIH
($90,000)
A Rapid Breath Volatile Metabolite-Based Diagnostic for In Vivo Identification and Antibiotic Resistance Profiling of Bacterial Pathogens in Ventilator-Associated PneumoniaSophia Koo2018-2023
ANR
(125,000 euros)
Mechanistic evaluation of the interaction between Enterobacteriaceae and fungi in the context of gut inflammationMathis Lavie-Richard and Harry Sokol2020-2024
Research agreement with Moderna Inc
(60,000 euros)
Screen of mRNA-encoded candidates against in infectious modelsSkurnik David2020-2023
ANR
(580,000 euros)
TnSeq Vaccinology: from pathophysiology studies to multivalent vaccine designSkurnik DavidUnder Review
ANR/BMBF
(600,000 euros)
Broad spectrum Antibodies Against MDR and pan-resistant bacteriaSkurnik DavidUnder Review

Last 25 Publications

  1. Rigourd, V, Mouadh, B, Poupon, J, Langrand, J, Goutard, A, Droguet, C et al.. Chlorine Solutions for a Safe Method of Decontamination of Breast Pump Milk Collection Kits Before and After the Coronavirus Disease 2019 Pandemic. Front Nutr. 2021;8 :574311. doi: 10.3389/fnut.2021.574311. PubMed PMID:33748168 PubMed Central PMC7969643.
  2. Angoulvant, F, Ouldali, N, Yang, DD, Filser, M, Gajdos, V, Rybak, A et al.. Coronavirus Disease 2019 Pandemic: Impact Caused by School Closure and National Lockdown on Pediatric Visits and Admissions for Viral and Nonviral Infections-a Time Series Analysis. Clin Infect Dis. 2021;72 (2):319-322. doi: 10.1093/cid/ciaa710. PubMed PMID:33501967 PubMed Central PMC7314162.
  3. Skurnik, D, Rybak, A, Yang, DD, Pons, S, Guedj, R, Levy, C et al.. Unexpected lessons from the COVID-19 lockdowns in France: Low impact of school opening on common communicable pediatric airborne diseases. Clin Infect Dis. 2020; :. doi: 10.1093/cid/ciaa1899. PubMed PMID:33373441 PubMed Central PMC7799226.
  4. Yang, DD, Ouldali, N, Gajdos, V, Thomas-Sertillanges, R, Vasante, L, Skurnik, D et al.. Common pediatric respiratory infectious diseases may serve as an early predictor for SARS-CoV-2 new wave of infections. Clin Infect Dis. 2020; :. doi: 10.1093/cid/ciaa1359. PubMed PMID:32894752 PubMed Central PMC7499521.
  5. Sloan, SE, Szretter, KJ, Sundaresh, B, Narayan, KM, Smith, PF, Skurnik, D et al.. Clinical and virological responses to a broad-spectrum human monoclonal antibody in an influenza virus challenge study. Antiviral Res. 2020;184 :104763. doi: 10.1016/j.antiviral.2020.104763. PubMed PMID:32151645 .
  6. Aschard, H, Laville, V, Tchetgen, ET, Knights, D, Imhann, F, Seksik, P et al.. Genetic effects on the commensal microbiota in inflammatory bowel disease patients. PLoS Genet. 2019;15 (3):e1008018. doi: 10.1371/journal.pgen.1008018. PubMed PMID:30849075 PubMed Central PMC6426259.
  7. Ouldali, N, Bellêttre, X, Milcent, K, Guedj, R, de Pontual, L, Cojocaru, B et al.. Impact of Implementing National Guidelines on Antibiotic Prescriptions for Acute Respiratory Tract Infections in Pediatric Emergency Departments: An Interrupted Time Series Analysis. Clin Infect Dis. 2017;65 (9):1469-1476. doi: 10.1093/cid/cix590. PubMed PMID:29048511 .
  8. Aschard, H, Guillemot, V, Vilhjalmsson, B, Patel, CJ, Skurnik, D, Ye, CJ et al.. Covariate selection for association screening in multiphenotype genetic studies. Nat Genet. 2017;49 (12):1789-1795. doi: 10.1038/ng.3975. PubMed PMID:29038595 PubMed Central PMC5797835.
  9. Roux, D, Weatherholt, M, Clark, B, Gadjeva, M, Renaud, D, Scott, D et al.. Immune Recognition of the Epidemic Cystic Fibrosis Pathogen Burkholderia dolosa. Infect Immun. 2017;85 (6):. doi: 10.1128/IAI.00765-16. PubMed PMID:28348057 PubMed Central PMC5442610.
  10. Aschard, H, Tobin, MD, Hancock, DB, Skurnik, D, Sood, A, James, A et al.. Evidence for large-scale gene-by-smoking interaction effects on pulmonary function. Int J Epidemiol. 2017;46 (3):894-904. doi: 10.1093/ije/dyw318. PubMed PMID:28082375 PubMed Central PMC5837518.
  11. Guillard, T, Pons, S, Roux, D, Pier, GB, Skurnik, D. Antibiotic resistance and virulence: Understanding the link and its consequences for prophylaxis and therapy. Bioessays. 2016;38 (7):682-93. doi: 10.1002/bies.201500180. PubMed PMID:27248008 .
  12. Skurnik, D, Cywes-Bentley, C, Pier, GB. The exceptionally broad-based potential of active and passive vaccination targeting the conserved microbial surface polysaccharide PNAG. Expert Rev Vaccines. 2016;15 (8):1041-53. doi: 10.1586/14760584.2016.1159135. PubMed PMID:26918288 PubMed Central PMC4985264.
  13. Sokol, H, Leducq, V, Aschard, H, Pham, HP, Jegou, S, Landman, C et al.. Fungal microbiota dysbiosis in IBD. Gut. 2017;66 (6):1039-1048. doi: 10.1136/gutjnl-2015-310746. PubMed PMID:26843508 PubMed Central PMC5532459.
  14. Skurnik, D, Roux, D, Pons, S, Guillard, T, Lu, X, Cywes-Bentley, C et al.. Extended-spectrum antibodies protective against carbapenemase-producing Enterobacteriaceae. J Antimicrob Chemother. 2016;71 (4):927-35. doi: 10.1093/jac/dkv448. PubMed PMID:26747103 PubMed Central PMC4790622.
  15. Roux, D, Pons, S, Guillard, T, Ricard, JD, Pier, GB, Skurnik, D et al.. Impact of Drug Resistance on Virulence and Fitness of Bacterial Pathogens. Crit Care Med. 2016;44 (1):e50. doi: 10.1097/CCM.0000000000001330. PubMed PMID:26672941 .
  16. Skurnik, D, Clermont, O, Guillard, T, Launay, A, Danilchanka, O, Pons, S et al.. Emergence of Antimicrobial-Resistant Escherichia coli of Animal Origin Spreading in Humans. Mol Biol Evol. 2016;33 (4):898-914. doi: 10.1093/molbev/msv280. PubMed PMID:26613786 PubMed Central PMC5013867.
  17. Roux, D, Danilchanka, O, Guillard, T, Cattoir, V, Aschard, H, Fu, Y et al.. Fitness cost of antibiotic susceptibility during bacterial infection. Sci Transl Med. 2015;7 (297):297ra114. doi: 10.1126/scitranslmed.aab1621. PubMed PMID:26203082 .
  18. Roux, D, Cywes-Bentley, C, Zhang, YF, Pons, S, Konkol, M, Kearns, DB et al.. Identification of Poly-N-acetylglucosamine as a Major Polysaccharide Component of the Bacillus subtilis Biofilm Matrix. J Biol Chem. 2015;290 (31):19261-72. doi: 10.1074/jbc.M115.648709. PubMed PMID:26078454 PubMed Central PMC4521046.
  19. Lu, X, Skurnik, D, Pozzi, C, Roux, D, Cywes-Bentley, C, Ritchie, JM et al.. A Poly-N-acetylglucosamine-Shiga toxin broad-spectrum conjugate vaccine for Shiga toxin-producing Escherichia coli. mBio. 2014;5 (2):e00974-14. doi: 10.1128/mBio.00974-14. PubMed PMID:24667709 PubMed Central PMC3977355.
  20. Skurnik, D, Roux, D, Cattoir, V, Danilchanka, O, Lu, X, Yoder-Himes, DR et al.. Enhanced in vivo fitness of carbapenem-resistant oprD mutants of Pseudomonas aeruginosa revealed through high-throughput sequencing. Proc Natl Acad Sci U S A. 2013;110 (51):20747-52. doi: 10.1073/pnas.1221552110. PubMed PMID:24248354 PubMed Central PMC3870709.
  21. Angoulvant, F, Rouault, A, Prot-Labarthe, S, Boizeau, P, Skurnik, D, Morin, L et al.. Randomized controlled trial of parent therapeutic education on antibiotics to improve parent satisfaction and attitudes in a pediatric emergency department. PLoS One. 2013;8 (9):e75590. doi: 10.1371/journal.pone.0075590. PubMed PMID:24086581 PubMed Central PMC3784452.
  22. Skurnik, D, Roux, D, Aschard, H, Cattoir, V, Yoder-Himes, D, Lory, S et al.. A comprehensive analysis of in vitro and in vivo genetic fitness of Pseudomonas aeruginosa using high-throughput sequencing of transposon libraries. PLoS Pathog. 2013;9 (9):e1003582. doi: 10.1371/journal.ppat.1003582. PubMed PMID:24039572 PubMed Central PMC3764216.
  23. Roux, D, Gaudry, S, Khoy-Ear, L, Aloulou, M, Phillips-Houlbracq, M, Bex, J et al.. Airway fungal colonization compromises the immune system allowing bacterial pneumonia to prevail. Crit Care Med. 2013;41 (9):e191-9. doi: 10.1097/CCM.0b013e31828a25d6. PubMed PMID:23887232 .
  24. Lescat, M, Clermont, O, Woerther, PL, Glodt, J, Dion, S, Skurnik, D et al.. Commensal Escherichia coli strains in Guiana reveal a high genetic diversity with host-dependant population structure. Environ Microbiol Rep. 2013;5 (1):49-57. doi: 10.1111/j.1758-2229.2012.00374.x. PubMed PMID:23757130 .
  25. Cywes-Bentley, C, Skurnik, D, Zaidi, T, Roux, D, Deoliveira, RB, Garrett, WS et al.. Antibody to a conserved antigenic target is protective against diverse prokaryotic and eukaryotic pathogens. Proc Natl Acad Sci U S A. 2013;110 (24):E2209-18. doi: 10.1073/pnas.1303573110. PubMed PMID:23716675 PubMed Central PMC3683766.
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