Alain Charbit

Alain Charbit, PhD, is Research Director (DR1) from the CNRS since 2010. He was recruited CR1 by the CNRS in 1991. After 15 years at the Institut Pasteur (Paris), he joined in 1998 the « Faculté de Médecine Paris Descartes » where he is currently co-heading team 7 at the Institut Necker-Enfants Malades.
Alain Charbit is also Director of the Structure Fédérative de recherche (SFR) Necker (Inserm US24, CNRS UMR3633) https://www.necker.fr//sfr-necker/


Last News of the Lab

JClinMicro_AJ2021

High resolution typing of Staphylococcus epidermidis

High resolution typing of Staphylococcus epidermidis based on cgMLST to investigate the hospital spread of multidrug resistant clones. Jamet et ...
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A Sheath Phosphorylation control T6SS Assembly

Critical Role of a Sheath Phosphorylation Site On the Assembly and Function of an Atypical Type VI Secretion System. Ziveri ...
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Chronic Staphylococcus Aureus Lung Infection Correlates With an Increased Intracellular Persistence

Chronic Staphylococcus Aureus Lung Infection Correlates With Proteogenomic and Metabolic Adaptations Leading to an Increased Intracellular Persistence. Tan et al, ...
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Last 25 Publications

  1. Rytter, H, Jamet, A, Coureuil, M, Charbit, A, Ramond, E. Which Current and Novel Diagnostic Avenues for Bacterial Respiratory Diseases?. Front Microbiol. 2020;11 :616971. doi: 10.3389/fmicb.2020.616971. PubMed PMID:33362754 PubMed Central PMC7758241.
  2. Jamet, A, Guglielmini, J, Brancotte, B, Coureuil, M, Euphrasie, D, Meyer, J et al.. High-Resolution Typing of Staphylococcus epidermidis Based on Core Genome Multilocus Sequence Typing To Investigate the Hospital Spread of Multidrug-Resistant Clones. J Clin Microbiol. 2021;59 (3):. doi: 10.1128/JCM.02454-20. PubMed PMID:33328176 .
  3. Quereda, JJ, Morel, C, Lopez-Montero, N, Ziveri, J, Rolland, S, Grenier, T et al.. A role for Taok2 in Listeria monocytogenes vacuolar escape. J Infect Dis. 2020; :. doi: 10.1093/infdis/jiaa367. PubMed PMID:32582947 .
  4. Tan, X, Coureuil, M, Charbit, A, Jamet, A. Multitasking Actors of Staphylococcus aureus Metabolism and Virulence. Trends Microbiol. 2020;28 (1):6-9. doi: 10.1016/j.tim.2019.10.013. PubMed PMID:31753539 .
  5. Ramond, E, Jamet, A, Coureuil, M, Charbit, A. Pivotal Role of Mitochondria in Macrophage Response to Bacterial Pathogens. Front Immunol. 2019;10 :2461. doi: 10.3389/fimmu.2019.02461. PubMed PMID:31708919 PubMed Central PMC6819784.
  6. Ziveri, J, Chhuon, C, Jamet, A, Rytter, H, Prigent, G, Tros, F et al.. Critical Role of a Sheath Phosphorylation Site On the Assembly and Function of an Atypical Type VI Secretion System. Mol Cell Proteomics. 2019;18 (12):2418-2432. doi: 10.1074/mcp.RA119.001532. PubMed PMID:31578219 PubMed Central PMC6885697.
  7. Tan, X, Ramond, E, Jamet, A, Barnier, JP, Decaux-Tramoni, B, Dupuis, M et al.. Transketolase of Staphylococcus aureus in the Control of Master Regulators of Stress Response During Infection. J Infect Dis. 2019;220 (12):1967-1976. doi: 10.1093/infdis/jiz404. PubMed PMID:31420648 .
  8. Vidal, C, Moulin, F, Nassif, X, Galmiche, L, Borgel, D, Charbit, A et al.. Fulminant arterial vasculitis as an unusual complication of disseminated staphylococcal disease due to the emerging CC1 methicillin-susceptible Staphylococcus aureus clone: a case report. BMC Infect Dis. 2019;19 (1):302. doi: 10.1186/s12879-019-3933-3. PubMed PMID:30943907 PubMed Central PMC6446405.
  9. Tan, X, Coureuil, M, Ramond, E, Euphrasie, D, Dupuis, M, Tros, F et al.. Chronic Staphylococcus aureus Lung Infection Correlates With Proteogenomic and Metabolic Adaptations Leading to an Increased Intracellular Persistence. Clin Infect Dis. 2019;69 (11):1937-1945. doi: 10.1093/cid/ciz106. PubMed PMID:30753350 .
  10. Le Gallou, S, Zhou, Z, Thai, LH, Fritzen, R, de Los Aires, AV, Mégret, J et al.. A splenic IgM memory subset with antibacterial specificities is sustained from persistent mucosal responses. J Exp Med. 2018;215 (8):2035-2053. doi: 10.1084/jem.20180977. PubMed PMID:29959173 PubMed Central PMC6080908.
  11. Brauge, T, Faille, C, Sadovskaya, I, Charbit, A, Benezech, T, Shen, Y et al.. The absence of N-acetylglucosamine in wall teichoic acids of Listeria monocytogenes modifies biofilm architecture and tolerance to rinsing and cleaning procedures. PLoS One. 2018;13 (1):e0190879. doi: 10.1371/journal.pone.0190879. PubMed PMID:29320565 PubMed Central PMC5761963.
  12. Jamet, A, Charbit, A, Nassif, X. Antibacterial Toxins: Gram-Positive Bacteria Strike Back!. Trends Microbiol. 2018;26 (2):89-91. doi: 10.1016/j.tim.2017.11.003. PubMed PMID:29174101 .
  13. Ziveri, J, Tros, F, Guerrera, IC, Chhuon, C, Audry, M, Dupuis, M et al.. The metabolic enzyme fructose-1,6-bisphosphate aldolase acts as a transcriptional regulator in pathogenic Francisella. Nat Commun. 2017;8 (1):853. doi: 10.1038/s41467-017-00889-7. PubMed PMID:29021545 PubMed Central PMC5636795.
  14. Jamet, A, Touchon, M, Ribeiro-Gonçalves, B, Carriço, JA, Charbit, A, Nassif, X et al.. A widespread family of polymorphic toxins encoded by temperate phages. BMC Biol. 2017;15 (1):75. doi: 10.1186/s12915-017-0415-1. PubMed PMID:28851366 PubMed Central PMC5576092.
  15. Ghenassia, A, Gross, DA, Lorain, S, Tros, F, Urbain, D, Benkhelifa-Ziyyat, S et al.. Intradermal Immunization with rAAV1 Vector Induces Robust Memory CD8+ T Cell Responses Independently of Transgene Expression in DCs. Mol Ther. 2017;25 (10):2309-2322. doi: 10.1016/j.ymthe.2017.06.019. PubMed PMID:28720467 PubMed Central PMC5628776.
  16. Barel, M, Charbit, A. Role of Glycosylation/Deglycolysation Processes in Francisella tularensis Pathogenesis. Front Cell Infect Microbiol. 2017;7 :71. doi: 10.3389/fcimb.2017.00071. PubMed PMID:28377902 PubMed Central PMC5359314.
  17. Rigard, M, Bröms, JE, Mosnier, A, Hologne, M, Martin, A, Lindgren, L et al.. Francisella tularensis IglG Belongs to a Novel Family of PAAR-Like T6SS Proteins and Harbors a Unique N-terminal Extension Required for Virulence. PLoS Pathog. 2016;12 (9):e1005821. doi: 10.1371/journal.ppat.1005821. PubMed PMID:27602570 PubMed Central PMC5014421.
  18. Pizarro-Cerdá, J, Charbit, A, Enninga, J, Lafont, F, Cossart, P. Manipulation of host membranes by the bacterial pathogens Listeria, Francisella, Shigella and Yersinia. Semin Cell Dev Biol. 2016;60 :155-167. doi: 10.1016/j.semcdb.2016.07.019. PubMed PMID:27448494 PubMed Central PMC7082150.
  19. Barel, M, Harduin-Lepers, A, Portier, L, Slomianny, MC, Charbit, A. Host glycosylation pathways and the unfolded protein response contribute to the infection by Francisella. Cell Microbiol. 2016;18 (12):1763-1781. doi: 10.1111/cmi.12614. PubMed PMID:27185209 .
  20. Brissac, T, Ziveri, J, Ramond, E, Tros, F, Kock, S, Dupuis, M et al.. Gluconeogenesis, an essential metabolic pathway for pathogenic Francisella. Mol Microbiol. 2015;98 (3):518-34. doi: 10.1111/mmi.13139. PubMed PMID:26192619 .
  21. Barel, M, Ramond, E, Gesbert, G, Charbit, A. The complex amino acid diet of Francisella in infected macrophages. Front Cell Infect Microbiol. 2015;5 :9. doi: 10.3389/fcimb.2015.00009. PubMed PMID:25705612 PubMed Central PMC4319460.
  22. Renier, S, Chafsey, I, Chambon, C, Caccia, N, Charbit, A, Hébraud, M et al.. Contribution of the multiple Type I signal peptidases to the secretome of Listeria monocytogenes: deciphering their specificity for secreted exoproteins by exoproteomic analysis. J Proteomics. 2015;117 :95-105. doi: 10.1016/j.jprot.2015.01.007. PubMed PMID:25637307 .
  23. Ramond, E, Gesbert, G, Guerrera, IC, Chhuon, C, Dupuis, M, Rigard, M et al.. Importance of host cell arginine uptake in Francisella phagosomal escape and ribosomal protein amounts. Mol Cell Proteomics. 2015;14 (4):870-81. doi: 10.1074/mcp.M114.044552. PubMed PMID:25616868 PubMed Central PMC4390266.
  24. Jamet, A, Jousset, AB, Euphrasie, D, Mukorako, P, Boucharlat, A, Ducousso, A et al.. A new family of secreted toxins in pathogenic Neisseria species. PLoS Pathog. 2015;11 (1):e1004592. doi: 10.1371/journal.ppat.1004592. PubMed PMID:25569427 PubMed Central PMC4287609.
  25. Gesbert, G, Ramond, E, Tros, F, Dairou, J, Frapy, E, Barel, M et al.. Importance of branched-chain amino acid utilization in Francisella intracellular adaptation. Infect Immun. 2015;83 (1):173-83. doi: 10.1128/IAI.02579-14. PubMed PMID:25332124 PubMed Central PMC4288867.
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